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Abstract
A simple micellar electrokinetic capillary chromatography is described for the determination of cyclosporin A in commercial preparation. The analysis of cyclosporin A was performed in a Tris buffer (10 mM; pH 7.1) with sodium dodecyl sulfate (SDS) (30 mM) as an anionic surfactant. Applied voltage was 20 kV and temperature was 25°C. Cefaclor was used as an internal standard and detection set at 200 nm. Several parameters affecting the separation of the drug were investigated, including the pH of the buffer, the concentrations of the buffer and SDS.
The linear range of the method for the determination of cyclosporin A was over 30–500 μM. Application of the method to the determination of cyclosporin A in capsules from a commercial source proved to be satisfactory.
ACKNOWLEDGMENTS
The authors are grateful to the Kaohsiung Medical University and National Science Council, Republic of China, for financial support of this work.