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Journal of Liquid Chromatography & Related Technologies Volume 23, 2000 - Issue 18
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Original Articles

DETERMINATION OF GLUCOSAMINE IN NUTRITIONAL SUPPLEMENTS BY REVERSED-PHASE ION-PAIRING HPLC

Wayne K. Way Chemir/Polytech Laboratories, Inc., 2672 Metro Blvd., Maryland Heights, MO, 63043, U.S.A.
,
Kathleen G. Gibson Chemir/Polytech Laboratories, Inc., 2672 Metro Blvd., Maryland Heights, MO, 63043, U.S.A.
&
Andrew G. Breite Chemir/Polytech Laboratories, Inc., 2672 Metro Blvd., Maryland Heights, MO, 63043, U.S.A.
Pages 2861-2871 | Received 01 May 2000, Accepted 27 May 2000, Published online: 02 Jun 2007
 
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Abstract

A reverse-phase high performance liquid chromatography (HPLC) method for the determination of glucosamine in nutritional supplements was developed and validated based on USP guidelines. Separations were performed using a MetaChem Inertsil ODS-3 column with an isocratic mobile phase consisting of 10% Methanol, 90% 0.005M octanesulfonate, pH = 2.1. Glucosamine was detected using a refractive index (RI) detector. The octanesulfonate ion pairing agent allowed for retention on a C-18 column.

The method proved highly reliable with respect to standard performance characteristics. The method allows for convenient quality control in the growing nutritional supplement market.

ACKNOWLEDGMENTS

This work was supported by Nutramax Laboratories®, Incorporated. Furthermore, we thank Jeff Niemeyer for his insightful discussions.

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