ENANTIOSEPARATIONS BY CAPILLARY ELECTROPHORESIS USING CHIRAL GLYCOSIDIC SURFACTANTS. II. COMPARISONOF CHIRAL CYCLOHEXYL-ALKYL-β-D-MALTOSIDE SURFACTANTS

Abstract

Three different glycosidic surfactants, namely cyclohexyl-butyl-β-D-maltoside (CYMAL-4), cyclohexyl-pentyl-β-D-maltoside (CYMAL-5), and cyclohexyl-hexyl-β-D-maltoside (CYMAL-6), were compared in the enantioseparation of some dansyl amino acids and methylated tryptophans. The three CYMAL surfactants have the same chiral maltoside head group but differ in the length of the hydrophobic tail. Increasing the length of the hydrophobic tail seems to shift the optimum surfactant concentration for maximum enantioresolution towards lower concentration values. In order to extend the range of optimum surfactant concentration over which maximum enantioresolution can be achieved, mixed CYMAL surfactant systems were introduced and evaluated. They consisted of mixing CYMAL-6 with either cyclohexyl-methyl-β-D-maltoside (CYMAL-1) or cyclohexyl-ethyl-β-D-maltoside (CYMAL-2).

ACKNOWLEDGMENT

We gratefully acknowledge the financial support by the Cooperative State Research, Education and Extension Service, U. S. Department of Agriculture under agreement No. 94-37102-0989 and Agreement No. 98-35102-6529.