COMPARISON OF LYSOZYME ADSORPTION TO IMMOBILIZED CIBACRON BLUE 3GA USING VARIOUS MEMBRANE SUPPORTS

Abstract

Lysozyme adsorption to immobilized Cibacron Blue 3GA using different membrane supports, regenerated cellulose membrane discs, and polysulfone hollow fibers, is investigated in this study. The relevant adsorption properties using traditional chromatographic beads were also measured for comparison. The experimental results demonstrated distinct adsorption properties for different solid supports. The membrane discs possessed the best thermal resistance among the three adsorbents assessed, which essentially results from their higher thermal stability. Moreover, the saturation capacity of lysozyme onto membrane discs was found close to the ligand density for gel beads, whereas the hollow fibers were of much greater saturation capacity.

ACKNOWLEDGMENT

This research was sponsored by the National Science Council of the Republic of China (NSC 85-2214-E-005-009 and NSC 86-2214-E-005-004).