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Abstract
We describe a new method for the analysis of hypericins from crude extracts of Hypericum perforatum (St. John’s Wort) using HPLC. Hypericin and pseudohypericin and related compounds were separated in 15 minutes using a simple isocratic elution method on a modified phenyl column. This isocratic method permits the quantitative and rapid analysis of hypericins in crude acetone samples.
In addition, we report on the photoconversion of protohypericins to hypericins, as well as the stability of hypericin and pseudohypericin as a function of light, time, and storage conditions.
ACKNOWLEDGMENTS
Financial support for Tara Sirvent was provided by a National Science Foundation Graduate Research Fellowship. We gratefully acknowledge insightful discussions with Stuart Krasnoff, Cornell University; Nan Vance and Loren Walker, USDA, Forest Service, Corvalis, Oregon. Mention of a trademark, proprietary products, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other vendors that may be suitable.