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Journal of Liquid Chromatography & Related Technologies Volume 23, 2000 - Issue 2
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Original Articles

DETERMINATION OF 2,6-PYRIDINEDICARBOXYLIC ACID IN PEACH JUICE

T. Cecchi Dipartimento di Scienze Chimiche, Università degli Studi di Camerino, Via S. Agostino 1, Camerino, 62032, Italy
,
S. Ferraro Dipartimento di Scienze Chimiche, Università degli Studi di Camerino, Via S. Agostino 1, Camerino, 62032, Italy
,
F. Fuscà Dipartimento di Scienze Chimiche, Università degli Studi di Camerino, Via S. Agostino 1, Camerino, 62032, Italy
,
F. Pucciarelli Dipartimento di Scienze Chimiche, Università degli Studi di Camerino, Via S. Agostino 1, Camerino, 62032, Italy
&
P. Passamonti Dipartimento di Scienze Chimiche, Università degli Studi di Camerino, Via S. Agostino 1, Camerino, 62032, Italy
Pages 313-319 | Received 25 Jan 1999, Accepted 04 Jun 1999, Published online: 06 Feb 2007
 
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Abstract

The quantitation of 2,6-pyridinedicarboxylic acids (2,6-PDA) in fruit juice can be achieved rapidly utilizing High Pressure Liquid Chromatography (HPLC).

The best chromatographic performance was obtained with an aqueous mobile phase containing 15 mM tetrabutylammonium phosphate and 2 mM EDTA; the pH was maintained at 7.2 by 10 mM phosphate buffer.

The influence of the eluent parameters on retention of PDAs has been investigated in order to elucidate the separation mechanisms involved in the ion pair chromatography of these ionizable substances.

Acknowledgments

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