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Original Articles

SEPARATION OF THE BRYOSTATIN DERIVATIVES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

, , , , &
Pages 399-409 | Received 12 Apr 1999, Accepted 26 Apr 1999, Published online: 06 Feb 2007
 

Abstract

The Separation of bryostatin derivatives (26-acetate, 26-metabromobenzoate, 26-ketone, and 13→ 30 epoxide) by high performance liquid chromatography (HPLC) using normal phase and reverse phase systems was accomplished.

Using n-hexane/EtOAc/MeOH/H2O (26:5:1:0.01) as mobile phase for normal phase HPLC and acetonitrile (CH3CN)/H2O system for reverse phase HPLC provided good separation of bryostatin derivatives. In addition, contributions of functional group for eluate order were discussed.

Acknowledgments

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