![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A rapid and simple microscale method for the determination of octanol-water partition coefficients has been developed and evaluated for a series of twenty-six bioactive 2-amino-2-oxazolines. Solutes were equilibrated between n-octanol and water by using the shake-flask approach in a single partitioning. The concentration of each compound was measured in the aqueous phase before and after partitioning directly by RP-HPLC, and the peak areas ratio was used to calculate the partition coefficient log P.
Log P values ranging from -0.22 to 3.69 have been determined with a precision better than ± 0.06 log unit. They were compared with the measured shake-flask octanol-water partition coefficient and with the calculated incremental Clog P values.
The linear regression analyses between these lipophilic parameters show significant correlations, allowing validation of this chromatographic approach for the log P determination.