45
Views
0
CrossRef citations to date
0
Altmetric
Original Articles

COMPARISON OF DIALYSIS AND GEL PERMEATION CHROMATOGRAPHY FOR ANTIGEN PURIFICATION

, &
Pages 489-496 | Received 27 Jun 1999, Accepted 20 Jul 1999, Published online: 06 Feb 2007
 

Abstract

Immunoaffinity chromatography can be applied to the treatment of biological samples. It consists of the separation, extraction, or purification of a molecule (para nitro analine in this paper) from a mixture by a selective interaction with an antibody. The latter is produced by living organisms after their contact with an antigen (modified protein in this case). The production of specific antibodies highly depends on the purity of antigen. This paper describes and compares two methods of antigen purification: dialysis and gel permeation chromatography. This chromatographic method allows one to obtain a pure antigen faster than dialysis but with a slightly larger dilution of the samples. It is not a drawback for the procedure of the antibodies production.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 583.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.