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Original Articles

PURIFICATION OF PEG-PROTEIN CONJUGATES BY COUNTERCURRENT DISTRIBUTION IN AQUEOUS TWO-PHASE SYSTEMS

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Pages 497-503 | Received 07 Jun 1999, Accepted 26 Jun 1999, Published online: 06 Feb 2007
 

Abstract

Purification of PEG-protein conjugates by countercurrent distribution (CCD) in aqueous two-phase systems is reported. By using CCD in 19.36% PEG 3400/14.68% potassium phosphate, we can separate PEG-lysozyme conjugates, which contains an average number of 1.14 PEG per lysozyme, into three peaks with 55 number of transfers. Further analysis of the samples reveals that these three peaks mainly represent unmodified lysozyme, (PEG)1-lysozyme and (PEG)2-lysozyme.

ACKNOWLEDGMENT

One of the authors, Terasut Sookkumnerd, is greatly grateful to Suranaree University of Technology, Thailand and Ministry of University Affairs, Royal Thai Government for providing financial support under the Faculty Development Program during his study at Lehigh University.

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