31
Views
2
CrossRef citations to date
0
Altmetric
Original Articles

EXTRACTION AND QUANTIFICATION OF BENZO[a]PYRENE IN SOIL BY REVERSED PHASE THIN LAYER CHROMATOGRAPHY

, , &
Pages 579-586 | Received 15 Jun 1999, Accepted 26 Jun 1999, Published online: 06 Feb 2007
 

Abstract

As research on the biodegradation of benzo[a]pyrene (B[a]P) shifts from laboratory to field based studies, a need has arisen for reproducible, economical, and accurate methods for extraction and quantification of B[a]P. This study describes a method for the analysis of B[a]P using batch extraction techniques with quantification by reversed phase high performance thin layer chromatography (HPTLC) and densitometry. A loam soil was fortified with B[a]P at a level of 100 mg B[a]P kg−1 soil. Several solvents were evaluated as potential soil extraction systems including: acetone, acetonitrile, ethyl acetate, methanol, and methylene chloride:acetone (1:1). Reverse phase thin layer chromatography of B[a]P was performed on hydrocarbon impregnated, C18 reverse phase plates using methanol:acetonitrile (1:1) as the developing solvent. The Rf for B[a]P in this system was found to be 0.52. Recovery of B[a]P from soil ranged from 11% with methanol to 84% with ethyl acetate.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 583.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.