Abstract
A rapid and simple reverse phase liquid chromatographic method has been developed for the simultaneous quantitation of anticancerous drugs vincristine, vinblastine and their precursors catharanthine and vindoline. Peak purity evaluation of these compounds in plant extract has also been studied using photo–diode array–UV detector.
ACKNOWLEDGEMENT
The authors are indebted to Prof. P. Potier (National Centre for Scientific Research, Yvette, Cedex, France), for the generous gift of vindoline and catharanthine standards.