DETERMINATION OF FUROSINE IN HYDROLYZATE OF PROCESSED MILK BY HPLC USING A NARROW BORE COLUMN AND DIODE-ARRAY DETECTOR

Abstract

A simple, rapid, sensitive, reproducible, and accurate HPLC procedure employing a narrow bore reversed phase column and a diode-array detector is proposed for the determination of ϵ-N-2-furoylmethyl-L-lysine (furosine) in acid hydrolyzed of milk and powdered milk samples.

After optimization of the variables involved, the method was characterized and validated in terms of selectivity, repeatability, sensitivity, and accuracy and applied to determine furosine content in hydrolyzed milk samples.

ACKNOWLEDGMENTS

We are grateful to Dr. G. Attioli (SAGIT, Division of Unilever Italia S.p.A., Cisterna di Latina, Italy) for the kind gift of samples of dried milks and dried milk whey. E. C. was the recipient of a postdoctoral fellowship from C.N.R. (National Research Council).