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Abstract
A high performance liquid chromatography method was developed for the determination of organic acids in commercial samples of Garcinia cambogia extracts. The major organic acid in commercially available extracts of Garcinia cambogia has been found to be (-) hydroxycitric acid present to the extent of 51–55%. Tartaric, citric, and malic acids are present in small quantities and have been detected by using 6 mM sulfuric acid as the mobile phase with a flow rate of 1.0 mL/min and UV detection at 210 nm.
This method is shown to estimate accurately (-) hydroxycitric acid in the samples of Garcinia cambogia extracts. Thus, the method is ideally suited for rapid routine analysis of commercial Garcinia cambogia extracts.