DEVELOPMENT OF HPLC METHOD FOR THE ANALYSIS OF CHLOROPHENOLS IN SAMPLES FROM ANAEROBIC REACTORS FOR WASTEWATER TREATMENT

Abstract

This article describes an HPLC method for routine samples analysis of laboratory scale anaerobic immobilized sludge reactors used for degradation of pentachlorophenol (PCP) in synthetic wastewater. The method was developed to quantify pentachlorophenol and six chlorophenol isomers produced during the degradation of pentachlorophenol by anaerobic bacteria. The validated method was used for anaerobic reactor samples and was able to quantify the chlorophenol intermediates produced.

ACKNOWLEDGMENTS

We would like to acknowledge Elizabeth Baraldi, from the Department of Civil Engineering - USP (São Carlos), for having supplied the samples for this work, Elizabeth M. Moraes and Maria Ângela Adorno for their participation in many stages of this work and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for financial support.