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Abstract
A simple and rapid method was developed for the determination of amfepramone hydrochloride, fenprorex, and diazepam in capsules using high performance liquid chromatography (HPLC) with UV detection. This procedure provided conditions for the separation of the active ingredient from the complex matrices of the dosage forms by extraction in methanol. Isocratic reversed phase chromatography was performed using acetonitrile, methanol, and aqueous 0,1% ammonium carbonate (50:10:40) as a mobile phase, LiChrospher 100 RP 18 column (125 × 5 mm id, 5μm), a column temperature of 25 ± 1°C and detection at 230 nm.
The calibration curves were linear over a wide concentration range (20–2000 μg.mL−1 to amfepramone hydrochloride, 8–800 μg.mL−1 to fenproporex, and 4–200 μg.mL−1 to diazepam) and good analytical recovery (87.1 to 107.8%) was obtained. The method is accurate and precise, as well as having advantages such as simplicity and short duration of analysis. Twenty samples of pharmaceutical preparations labelled as “natural” products were analysed. Anorectics and diazepam, were detected in 40% of the samples.
ACKNOWLEDGMENT
The authors would like to thank Professor Leonard Peach from Fac. Ciênc. Farm., UNESP, Doris Barnes for the English revision, and Elaine F. G. Jardim for technical support.