HIGH PRESSURE LIQUID CHROMATOGRAPHIC DETERMINATION OF NITRENDIPINE IN HUMAN PLASMA AFTER SOLID PHASE EXTRACTION

Abstract

A rapid and sensitive Reversed-Phase High Pressure Liquid Chromatographic (RP-HPLC) method has been developed for the quantitative determination of nitrendipine in human plasma.

A Hypersil C₁₈ 150×4.6 mm, 5 μm analytical column was used with a mixture of CH₃CN-H₂O at a volume ratio 50:50 v/v. Detection was performed with a variable wavelength UV-VIS detector at 238 nm. The detection limit was 0.4 ng/mL.

Nimodipine was used as internal standard (IS) at a concentration of 12 ng/mL.

The statistical evaluation of the method was examined performing intra-day and inter-day calibration.

Nitrendipine was extracted from human plasma matrices by Solid Phase Extraction (SPE) technique instead of conventional liquid-liquid extraction methods. Recovery of nitrendipine in spiked samples was 97.75±5.43.

The method was applied to a preliminary pharmacokinetic study in which an oral dose of 20 mg of nitrendipine was given to eight healthy volunteers.

Acknowledgments