Abstract
A micellar electrokinetic capillary chromatography method is presented to separate sulfamethazine, sulfaquinoxaline, menadione, and pyrimethamine. Separation was carried out at 20 kV for the five first minutes and then rised to 30 kV until the end came, using 30 mM borate buffer adjusted to pH 9.2, 6% acetonitrile, and 40 mM sodium dodecyl sulfate as electrolyte.
The limits of quantification were about 1 mg/L for every component. The method was applied in veterinary products and the results showed that some commercial claimed levels are not in agreement with the obtained results by using our analytical method, as are in other cases.
Finally, a HPLC method is used to determine the same mixture with similar results, than that provided by micellar electrokinetic capillary chromatography.
ACKNOWLEDGMENT
The authors are grateful to the DGES of the Ministerio de Educación y Cultura (Spain) for supporting this study (Project PB-97-0431-C03-01).