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Abstract
A new, simple, precise, accurate, rapid, and stability indicating high performance thin layer chromatography (HPTLC) method has been developed for the simultaneous determination of pyridoxine hydrochloride and doxylamine succinate in tablets. The stationary phase was silica gel 60F254 HPTLC plates and the mobile phase was acetone - chloroform - methanol - 25% ammonia solution (7 : 1.5 : 0.3 : 1.2, v/v). Detection and quantification was carried out densitometrically at 269 nm. The linearity ranges were 0.5–2.0 μg/spot for both pyridoxine hydrochloride and doxylamine succinate. Assays for pyridoxine hydrochloride and doxylamine succinate were 10.20 mg/tab (RSD 0.73%) and 10.00 mg/tab (RSD 1.93%) respectively for the brand analyzed. Percentage recoveries of pyridoxine hydrochloride and doxylamine succinate were in the range of 99.30–103.00% and 97.70–101.00%, respectively.