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Abstract
In this study, we reported a chromatographic protocol using C18 silica gel phase and basic Al2O3 phase to research the creating and recovery of taxol from the extract of Taxus cuspidate callus cultures. With the cleaning up by basic Al2O3 chromatography, the content of taxol determined in our experiment was increased greatly. The main source of increasing taxol concentration was the isomerization of 7-epi-taxol to taxol; taxol could also be decomposed to baccatin III and 10-deacetylbaccatin III in the Al2O3 column. At the optimized conditions, the total recovery of taxol from the extract was more than 160%.
ACKNOWLEDGMENT
The current address for Z. Zhang: State Key Laboratory of Microbial Resource, Institute of Microbiology, Chinese Academy of Science, Beijing, 100080, P. R. China.