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Journal of Liquid Chromatography & Related Technologies Volume 23, 2000 - Issue 20
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Original Articles

SELECTIVE CHEMILUMINESCENCE ANALYSIS OF AMADORI FORM OF GLYCATED HUMAN SERUM ALBUMIN

Toshihiko Hanai Health Research Foundation, Institut Pasteur 5F, Sakyo-ku, Kyoto, 606-8225, Japan
,
Miyuki Uchida School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo, 108-0072, Japan
,
Michiko Amao School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo, 108-0072, Japan
,
Chiaki Ikeda School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo, 108-0072, Japan
,
Kuniko Koizumi School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo, 108-0072, Japan
&
Toshio Kinoshita School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo, 108-0072, Japan
Pages 3119-3131 | Received 14 May 2000, Accepted 03 Jun 2000, Published online: 02 Nov 2011
 
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Abstract

Human serum albumin was purified by size-exclusion liquid chromatography. The glycated albumin in the effluent was selectively measured by a post-column reaction detector. The selective reaction mechanism is to measure the amount of Amadori form of glycated albumin. According to the Maillard reaction, glycosylated amines are rearranged to Amadori form ketoamines which can produce superoxide in strong alkaline solution, and this superoxide produces chemiluminescence with lucigenin. The chemiluminescence intensity was related to the concentration of glycated albumin in albumin while the fluorescence intensity was the same.

Acknowledgments

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