COLLECTION CAPACITY OF A SOLID PHASE TRAP IN SUPERCRITICAL FLUID EXTRACTION FOR THE EXTRACTION OF LIPIDS FROM A MODEL FAT SAMPLE

Abstract

Collection capacity of lipids from a model fat in supercritical fluid extraction using a solid phase traps was investigated. It was found that 0.6 g of an octadecylsilica material efficiently trapped 80–100 mg of fat. By developing a fractionated extraction/elution procedure, samples containing up to 500 mg of fat could easily be trapped. Losses due to the vapor pressure of the fat components were negligible, even after several hours of purging with gaseous carbon dioxide at a flow rate of ca 1 L/min. The trapping efficiency was found to be independent of the flow rate up to at least ca 1 L/min of gaseous carbon dioxide, and of modifiers (ethanol, methanol) in concentrations up to at least 10% if the temperature in the trap was sufficiently high to prevent modifier condensation.

ACKNOWLEDGMENTS

The authors gratefully acknowledge financial support from the Swedish Environmental Protection Agency and the Natural Science Research Council. Nils Nilsson is acknowledged for technical assistance.