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Journal of Liquid Chromatography & Related Technologies Volume 24, 2001 - Issue 14
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Original Articles

PREPARATIVE ISOLATION AND PURIFICATION OF PHENYLETHANOID GLYCOSIDES FROM THE EXTRACT OF FAECES OF BEAGLE DOGS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

Li Lei Department of Natural Medicines, School of Pharmaceutical Sciences, Peiking University, Beijing, 100083, P.R. China
,
Fuquan Yang Beijing Institute of New Technology Application, Beijing, 100035, P.R. China
,
Tianyou Zhang Beijing Institute of New Technology Application, Beijing, 100035, P.R. China
,
Pengfei Tu Department of Natural Medicines, School of Pharmaceutical Sciences, Peiking University, Beijing, 100083, P.R. China
,
Lijun Wu Shenyang Pharmaceutical University, Shenyang, 110015, P.R. China
,
Fakui Chen Shenyang Pharmaceutical University, Shenyang, 110015, P.R. China
&
Yoichiro Ito Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, U.S.A.Correspondence[email protected]
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Pages 2187-2195 | Received 15 Jan 2001, Accepted 10 Feb 2001, Published online: 06 Feb 2007
 
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Abstract

High-speed countercurrent chromatography (HSCCC) was applied to the separation of acteoside and isoacteoside from a crude extract from canine faeces after the dogs were orally administered phenylethanoid glycosides (PhGs). Two-step separation with a quaternary two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (system 1, 35:6:6:50, v/v; system 2, 30:10:6:50, v/v) was used. HPLC analysis of final CCC fractions revealed that the two main PhGs showed purity of over 95%. Their chemical structures were identified by 1HNMR, 13CNMR, FAB-MS.

ACKNOWLEDGMENTS

Financial support from Beijing Academy of Science & Technology and Beijing Commission of Science & Technology is gratefully acknowledged. We also thank senior engineer Xining Li for his excellent technical assistance.

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