SEPARATION OF CEFACLOR AND δ-3-CEFACLOR BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY

Abstract

A new micellar electrokinetic chromatography method for the separation of cefaclor and δ-3-cefaclor using sodium cholate (SC) as an anionic surfactant, 35% (v/v) of acetonitrile as organic modifier and 3-cyclohexylamino-1-propane sulfonic acid (CAPS) as buffer electrolyte was developed. The influences of buffer pH, organic modifiers (including methanol and acetonitrile), and different surfactants (sodium cholate, sodium deoxycholate, and sodium dodecyl sulfate) on the separation of cefaclor and δ-3-cefaclor were investigated. Calibration line and reproducibility of the developed method were examined. The method was applied to determine active ingredients in cefaclor for oral dry suspension. The results were satisfactory.

ACKNOWLEDGMENTS

Thanks are expressed to Shantou University “211 Project” and Shantou University Science Foundation for their financial support.