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Journal of Liquid Chromatography & Related Technologies Volume 25, 2002 - Issue 6
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Original Articles

VERSATILE TWO-PHASE SOLVENT SYSTEM FOR FLAVONOID PREFRACTIONATION BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

L. M. Yuan Department of Chemistry, Yunnan Normal University, Kunming, 650 092, P. R. ChinaCorrespondence[email protected]
,
P. Ai. Department of Chemistry, Yunnan Normal University, Kunming, 650 092, P. R. China
,
X. X. Chen Department of Chemistry, Yunnan Normal University, Kunming, 650 092, P. R. China
,
M. Zi. Department of Chemistry, Yunnan Normal University, Kunming, 650 092, P. R. China
,
P. Wu. Department of Chemistry, Yunnan Normal University, Kunming, 650 092, P. R. China
,
Z. Y. Li Department of Chemistry, Yunnan Normal University, Kunming, 650 092, P. R. China
&
Y. G. Chen Department of Chemistry, Yunnan Normal University, Kunming, 650 092, P. R. China
 show all
Pages 889-897 | Received 28 Aug 2001, Published online: 11 Dec 2006
 
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Abstract

High-speed countercurrent chromatography was used for the prefractionation of flavonoids from the crude extracts of Pericarpium citri reticulatae, Radix puerariae, Radix glycyrrhizae, Radix scutellariae, and Flos genkwa. All separations were performed only with a two-phase system composed of CHCl3 : CH3OH :H2O=4 : x : 2. The x was changed for each sample between 2.5∼4.5. The fractionated components were identified by thin-layer chromatography, which confirmed this solvent system was versatile and very useful for prefractionation of flavonoids.

ACKNOWLEDGMENTS

This work was supported by the National Natural Science Foundation and the Yunnan Province's, Natural Science Foundation of China.

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