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Journal of Liquid Chromatography & Related Technologies Volume 25, 2002 - Issue 6
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Original Articles

THE OPTIMIZATION OF THE SEPARATION CONDITIONS FOR CANNABINOIDS FROM CANNABIS SATIVAL.VAR INDICA AND APPLICATION OF THE METHOD TO DETERMINE THE CONTENT OF Δ9-TETRAHYDROCANNABINOL IN PLANT MATERIAL

E. Wojtasik Department of Plant Materials and Herbal Medicine, Drug Institute, 30/34 Chelmska, Str., Warsaw, 00725, Poland
,
M. Anyzewska Department of Plant Materials and Herbal Medicine, Drug Institute, 30/34 Chelmska, Str., Warsaw, 00725, Poland
&
I. Arent Department of Plant Materials and Herbal Medicine, Drug Institute, 30/34 Chelmska, Str., Warsaw, 00725, Poland
Pages 949-959 | Received 01 Nov 2001, Published online: 11 Dec 2006
 
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Abstract

The method for determination of isomer Δ9 of tetrahydrocannabinol in Cannabis sativa L. var indica has been developed. It is based on HPLC separation of isomers, Δ9 and Δ8, with resolution factor Rs=1.00. The method validation involved specificity and precision. Based on analysis of the raw material – marihuana, the variation coefficient RSD was found to be 2.09%.

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