Advanced search
Publication Cover
Journal of Liquid Chromatography & Related Technologies Volume 25, 2002 - Issue 12
Submit an article Journal homepage
152
Views
10
CrossRef citations to date
0
Altmetric
Original Articles

INVESTIGATION OF ALTERNATIVE REAGENTS TO 2-MERCAPTOETHANOL FOR THE PRE-COLUMN DERIVATIZATION OF FUMONISINS WITH O-PHTHALDIALDEHYDE, FOR HPLC ANALYSIS

Joerg Stroka Joint Research Centre of the European Commission, Food Products Unit, Ispra, I-21020, Italy
,
Claudia Capelletti Joint Research Centre of the European Commission, Food Products Unit, Ispra, I-21020, Italy
,
Andri Papadopoulou-Bouraoui Joint Research Centre of the European Commission, Food Products Unit, Ispra, I-21020, Italy
,
Lea Pallaroni Joint Research Centre of the European Commission, Food Products Unit, Ispra, I-21020, Italy
&
Elke Anklam Joint Research Centre of the European Commission, Food Products Unit, Ispra, I-21020, ItalyCorrespondence[email protected]
Pages 1821-1833 | Received 01 Mar 2002, Accepted 10 Apr 2002, Published online: 06 Feb 2007
 
Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days

ABSTRACT

This paper investigates the suitability of 37 reaction partners for the derivatization of fumonisins with o-phthaldialdehyde (OPA). All resulting fumonisin derivatives were compared for stability by UV-absorption. Subsequently, the relative fluorescence and retention times of derivatives found to be stable in UV-absorption were investigated by high performance liquid chromatography (HPLC), using well-established chromatographic conditions for fumonisin analysis. Several alternatives to 2-mercaptoethanol could be identified to be superior derivatization agents (3-mercapto-propionic acid, N-acetyl-cystein and thioglycerol). Those are especially valid when immunoaffinity column clean up is used prior to derivatization. The structure of the fumonisin B1-derivative obtained from the most suitable derivatization agent N-acetyl-cystein was verified by HPLC-MS.

Acknowledgments

Log in via your institution

Access through your institution

Log in to Taylor & Francis Online

Restore content access

Restore content access for purchases made as guest
Purchase options * Save for later

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 583.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.