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Journal of Liquid Chromatography & Related Technologies Volume 25, 2002 - Issue 3
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Original Articles

DETERMINATION OF CIPROFLOXACIN AND ITS METABOLITES IN URINE, USING ON-LINE SPE

D. H. Kim Department of Chemistry, Yonsei University, Seoul, 120-749, Korea
,
S. K. Lee Computer Aided Molecular Design Research Center, Soongsil University, Seoul, 156-743, Korea
,
Y. H. Park Lab Solution, Inc., NKIC 323, 48-84 Hongeundong, Seodaemoongu, Seoul, 120-749, Korea
&
D. W. Lee Department of Chemistry, Yonsei University, Seoul, 120-749, KoreaCorrespondence[email protected]
Pages 433-443 | Received 27 Jul 2001, Accepted 24 Aug 2001, Published online: 16 Aug 2006
 
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Abstract

An isocratic high-performance liquid chromatographic method with column switching and a direct injection system was developed to determine ciprofloxacin, a kind of quinolone derivative and its four kinds of metabolites in urine. A manually actuated system equipped with six port valves allowed a clean-up step with 0.01 M NaH2PO4 (pH 7.0) and the transfer of the analyte by a backflush mode. Also, the sample pretreatment condition for spiked urine was investigated to determine the optimum SPE condition for all analytes. All analytes showed over 92% recovery, using C18(10 μm, 30−50 μm), XAD-2 and XAD-4 as precolumn.

ACKNOWLEDGMENT

This work was supported by KOSEF.

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