CHIRAL RESOLUTION OF CROMAKALIM BY HPLC ON TEICOPLANIN AND TEICOPLANIN AGLYCON CHIRAL STATIONARY PHASES

ABSTRACT

The chiral resolution of cromakalim has been achieved on macrocyclic glycopeptide antibiotics columns, namely teicoplanin and teicoplanin aglycon known as Chirobiotic T and Chirobiotic TAG, respectively. The mobile phases used were hexane–ethanol (95 : 5, v/v) on Chirobiotic T and hexane–ethanol (75 : 25, v/v) on Chirobiotic TAG columns, respectively, at a flow rate of 0.5 mL/min. The detection was carried out at 220 nm. The α values of the resolved enantiomers were 1.13 and 1.12 on Chirobiotic T and Chirobiotic TAG columns, respectively. The values of Rs were 1.27 and 1.25 on Chirobiotic T and Chirobiotic TAG columns, respectively. The detection of the enantiomers was better on Chirobiotic T column than on Chirobiotic TAG column.

Keywords:

• Cromakalim
• Stereoselective chromatography
• Teico-planin
• Teicoplanin aglycon
• Macrocyclic glycopeptide antibiotics

ACKNOWLEDGMENT

The authors (I.A. and H.Y.A-E.) would like to thank the King Faisal Specialist Hospital and Research Center, Riyadh, administration for their support for the Pharmaceutical Analysis Laboratory Research Program.