Abstract
A reversed‐phase high performance liquid chromatographic (HPLC) method was developed for the simultaneous analysis of a group of fourteen positional isomeric di‐ and tri‐substituted benzenes; including o‐, m‐, and p‐aminophenol, o‐, m‐, and p‐nitrophenol, o‐, m‐, and p‐nitroaniline, 2‐chloro‐4‐nitroaniline, 4‐chloro‐2‐nitroaniline, 2,4‐dinitroaniline, 2,6‐dinitroaniline, and 3,5‐dinitroaniline. Baseline separation was achieved within 40 min using a Cyclobond I RSP (R,S‐hydroxypropyl ether‐β‐cyclodextrin) stationary phase in conjunction with gradient elution with methanol–water as the mobile phase. All compounds were detected at 254 nm. The quantitation limits ranged from 0.80 to 5.6 ng/mL.
Acknowledgments
Verónica Pino acknowledges the Spanish Ministry of Education and Culture for a F. P. U. Fellowship Grant. Partial support of this work by a Wake Forest University Research and Publication Fund Grant and by Advanced Separation Technologies, Inc., Whippany, NJ, is gratefully acknowledged.