Separate Determination of Rate Constants from Reversible Reactions in a Chromatographic Column and Eluent Using Empirical Peak Shape Equations

Abstract

A new mathematical‐analytical equation is derived for fitting purposes in the determination of mobile phase rate constants of enantiomerization from liquid chromatographic reactor experiments with a recycling of the internal chromatogram via an empty capillary. The equation was successfully tested with results from numerical calculations, as well as results from the on‐column enantiomerization of oxazepam.

Keywords:

• Chromatographic reactor
• Reversible reactions
• Rate constants

Acknowledgment

The authors gratefully acknowledge financial support from the Deutsche Forschungsgemeinschaft (Proj. HA 2238/3‐1).