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Journal of Liquid Chromatography & Related Technologies Volume 26, 2003 - Issue 6
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Original Articles

Determination of Acetaminophen and Phenacetin in Porcine Microsomal Samples

Sherry K. Cox Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, Tennessee, 37901, USACorrespondence[email protected]
,
Tina Hamner Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, Tennessee, 37901, USA
&
Joe Bartges Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee, USA
Pages 925-935 | Received 13 Oct 2002, Accepted 20 Nov 2002, Published online: 24 Jun 2011
 
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Abstract

A simple, quick, and sensitive high performance liquid chromatography (HPLC) analysis of phenacetin and acetaminophen in porcine hepatic microsome samples is described. Chromatography was performed on a YMC‐Pack ODS‐AQ column using a gradient mobile phase consisting of 0.05% phosphoric acid (pH 3):methanol. UV detection was measured at 254 nm. The procedure produced a linear curve for the concentration range 10–1500 ng/mL and had a limit of detection of 5 ng/mL for both compounds. This assay produced accurate and repeatable quantification of acetaminophen and phenacetin.

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