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Journal of Liquid Chromatography & Related Technologies Volume 26, 2003 - Issue 6
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Original Articles

HPLC Determination of Sulfathiazole in French Honeys

Anne‐Claire Martel Secteur des Contaminants Physico‐Chimiques, Agence Française de Sécurité Sanitaire des Aliments (AFSSA) Site de Sophia Antipolis, Laboratoire d'Études et de Recherches sur les Petits Ruminants et les Abeilles (LERPRA), 105 route des Chappes, F‐06902, Sophia Antipolis, Cedex, FranceCorrespondence[email protected]
&
Sarah Zeggane Secteur des Contaminants Physico‐Chimiques, Agence Française de Sécurité Sanitaire des Aliments (AFSSA) Site de Sophia Antipolis, Laboratoire d'Études et de Recherches sur les Petits Ruminants et les Abeilles (LERPRA), 105 route des Chappes, F‐06902, Sophia Antipolis, Cedex, France
Pages 953-961 | Received 15 Oct 2002, Accepted 22 Nov 2002, Published online: 24 Jun 2011
 
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Abstract

A rapid reversed‐phase high‐performance liquid chromatographic method, with fluorimetric detection, is described for routine analysis of sulfathiazole (STZ) residues in honey. Samples were dissolved in 1 M hydrochloric acid and submitted to pre‐column derivatization with fluorescamine. Liquid chromatographic analysis of the fluorescent derivative is performed on a C18 column using a mobile phase of 2% acetic acid/ acetonitrile (60:40, v/v). The quantification limit obtained for STZ in honey was 10 µg/kg.

Acknowledgment

We thank DGAL (Ministry of Agriculture) for help in the national sampling and the financial support.

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