Advanced search
Publication Cover
Journal of Liquid Chromatography & Related Technologies Volume 26, 2003 - Issue 6
Submit an article Journal homepage
30
Views
3
CrossRef citations to date
0
Altmetric
Original Articles

Simple HPLC Method for the Determination of Enalaprilat in Human Plasma

Laila I. Abou‐Basha National Organization for Drug Control and Research, Cairo, Egypt
,
Lobna F. Wahman National Organization for Drug Control and Research, Cairo, Egypt
&
Hassan Y. Aboul‐Enein Pharmaceutical Analysis Laboratory, Biological and Medical Research, Department (MBC 03‐65), King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh, 11211, Saudi ArabiaCorrespondence[email protected]
Pages 987-991 | Received 27 Oct 2002, Accepted 29 Nov 2002, Published online: 06 Feb 2007
 
Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days

Abstract

A simple and reliable isocratic reversed phase high performance liquid chromatography (HPLC) method for the determination of enalaprilat in human plasma is described. After plasma extraction, enalaprilat is analyzed using a µ Bondapak C18 column kept at 50°C. The mobile phase consists of 60% A (0.01 M KH2PO4, pH 4.0) and 40% B (80% of 50:50 CH3CN:CH3OH + 20% A) v/v. Enalaprilat is monitored by UV detection at 205 nm. The method is quite simple and sufficiently sensitive, with a limit of quantitation of 5 ng/mL and limit of detection of 1 ng/mL; within day and between‐days assays showed a relative standard deviation (RSD) below 15%.

Log in via your institution

Access through your institution

Log in to Taylor & Francis Online

Restore content access

Restore content access for purchases made as guest
Purchase options * Save for later

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 583.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.