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Original Articles

Comparison of Different Capillary Electrophoresis Modes and HPLC for the Separation of Xanthones

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Pages 993-1003 | Received 22 Oct 2002, Accepted 19 Nov 2002, Published online: 24 Jun 2011
 

Abstract

Nine xanthones from a Chinese traditional medicine, Securidaca inappendiculata Hassk, were separated by different capillary electrophoresis (CE) modes, including capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), microemulsion electrokinetic capillary chromatography (MEEKC), and capillary electrochromatography (CEC). The comparison of separation selectivity and efficiency for xanthones by using these CE methods indicated that different CE modes greatly varied in the separation selectivity and efficiency for xanthones; their advantages and disadvantages are discussed. Moreover, compared with traditional chromatographic methods, e.g., high performance liquid chromatography (HPLC), CE shows higher separation ability and analytical speed for these xanthones.

Acknowledgments

The authors would like to acknowledge Agilent Technologies for providing the capillary electrophoresis system and the CEC column. The technical assistance of X. D. Yang is also appreciated. The study is supported by NSFC, Grant No. 20275001.

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