Separation of Selected Bile Acids by TLC. I

Abstract

Seven of the selected bile acids were investigated: cholic acid (C), chenodeoxycholic acid (CDC), deoxycholic acid (DC), lithocholic acid (LC), glycocholic acid (GC), glycodeoxycholic acid (GDC), and glycolithocholic acid (GLC). The retardation factor (R _F ), ΔR _F values, the separation factors (α), resolution factors (R _S ), as well as the constants of the pair separation (R _F ^α), indicate that the mobile phase n‐heptane–ethyl acetate–acetic acid about volume composition 25:20:8, is the best for separations of investigated bile acids on aluminum plates precoated silica gel 60 F₂₅₄.

Keywords:

• Bile acids
• TLC