Capillary Electrophoretic Analysis of Pharmacologically Active Xanthone Compounds from Swertia przewalskii pissjauk Extract

Abstract

Pharmacologically active xanthone compounds isolated from Swertia przewalskii pissjauk were well separated by capillary electrophoresis (CE) within 5 min, using a running buffer of 25 mM disodium tetraborate at pH 9.0. Quantitative determination was shown to be possible because regression equations revealed a linear relationship between the peak area of each constituent and its concentration, with correlation coefficients of 0.9972–0.9994. The relative standard deviations were between 0.44%–0.73% for migration times and 2.52%–4.28% for peak areas. The dissociation constant of 1,7‐O‐β‐D‐glucopyranosyl‐8‐hydroxy‐3,7‐dimethoxyxanthone, 1,8‐dihydroxy‐3, 7‐dimethoxy‐xanthone and 1,7‐di‐hydroxy‐3,8‐dimethoxyxanthone were also measured by the CE method, giving a value of 9.04, 8.94, and 8.59, respectively.

Keywords:

• Capillary electrophoresis
• Xanthones
• Swertia przewalskii

Acknowledgments

This work was financially supported by National Sciences Foundation of China (No. 29825112) and The Chinese Academy of Sciences (No. KJ951‐A1‐507).