A Rapid and Sensitive HPLC Method for the Determination of Venlafaxine and O‐Desmethylvenlafaxine in Human Plasma with UV Detection

Abstract

A rapid and sensitive high performance liquid chromatographic (HPLC) method was developed and validated for the analysis of venlafaxine (VEN) and O‐desmethylvenlafaxine (ODV) in human plasma. The analytes were extracted from human plasma by using liquid–liquid extraction technique. Citalopram (CTP) was used as the internal standard. A Zorbax XDB C‐18 column provided chromatographic separation of analytes followed by UV detection with wavelength at 229 nm. The linear range for both analytes was 5 ng/mL to 1000 ng/mL with precision and accuracy of <10%. Total elution time was 12 min.

Keywords:

• Venlafaxine
• ODV
• Plasma
• Citalopram

Acknowledgments

The authors are thankful to Dr. P. K. Deshpande, Wockhardt Research Centre, for his help in the synthesis of ODV. The authors are also thankful to Wockhardt Research Centre, Aurangabad and Head—Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad for providing the facilities for this research work.