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Journal of Liquid Chromatography & Related Technologies Volume 26, 2003 - Issue 9-10
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Original Articles

Separation of Dammarane‐Saponins from Notoginseng, Root of Panax notoginseng (Burk.) F. H. Chen, by HSCCC Coupled with Evaporative Light Scattering Detector

Xue‐Li Cao Beijing Institute of New Technology Application, Xizhimen South Street, No. 16, Beijing, 100035, P.R. China
,
Yu Tian Beijing Institute of New Technology Application, Xizhimen South Street, No. 16, Beijing, 100035, P.R. China
,
Tian‐You Zhang Beijing Institute of New Technology Application, Xizhimen South Street, No. 16, Beijing, 100035, P.R. ChinaCorrespondence[email protected]
,
Qing‐Hui Liu Beijing Institute of New Technology Application, Xizhimen South Street, No. 16, Beijing, 100035, P.R. China
,
Li‐Jun Jia Beijing Institute of New Technology Application, Xizhimen South Street, No. 16, Beijing, 100035, P.R. China
&
Yoichiro Ito Biophysical Chemical Laboratory, Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Pages 1579-1591 | Received 21 Apr 2002, Accepted 07 Oct 2002, Published online: 06 Feb 2007
 
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Abstract

High‐speed countercurrent chromatography (HSCCC) coupled with evaporative light scattering detection, was applied to the separation of saponins from notoginseng, the main root of Panax notoginseng (Burk.) F. H. Chen. Five individual dammarane saponins have been isolated with the solvent systems composed of CHCl3/MeOH/2‐BuOH/H2O (5:6:1:4, v/v/v/v) and EtOAc/1‐BuOH/H2O (1:1:2, v/v/v), successively. They were identified as ginsenoside‐Rg1, ginsenoside‐Rd, notoginsenoside‐R1, ginsenoside‐Re, and ginsenoside‐Rb1 by FAB‐MS and 13C NMR, together with HPLC and TLC analysis.

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