HPLC Method for the Analysis of Atrazine in Freshwater Bivalves

Abstract

A method for the analysis of atrazine in freshwater bivalves has been developed. The method involves a simple liquid–liquid extraction procedure followed by high‐performance liquid chromatography analysis using a C₁₈ column, a methanol–water (60:40, v/v) mobile phase, a flow‐rate of 1 mL/min, and UV detection at 230 nm. After optimization of the conditions, the method was validated, showing 81.7% atrazine recovery. Performance data for the method such as linearity, precision, and quantitation limit are also reported.

Keywords:

• Atrazine
• HPLC
• Bivalves

Acknowledgments

The authors are grateful to FAPESP (Fundação de Amparo a Pesquisa do Estado de São Paulo) and CAPES for financial support, to Chemistry Department, FFCLRP, for the HPLC equipment, and to Programa de Pös Graduaµa¬o em Biologia Comparada. The authors are also grateful to Mércia Virgínia Carlos for technical support.