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Abstract
The chromatographic performance of 1.5 cm I.D. convoluted teflon tubing was studied at low rotary speeds ranging from 34 to 105 rpm. Results were as follows: (i) many solvent systems showed excellent retention of the stationary phase even at a high flow rate of the mobile phase; (ii) a good mixing of the two phases gave efficient solute partitioning resulting in high peak resolution; (iii) increasing the sample size caused only minor peak broadening; and (iv) increase in peak width and resolution using longer columns is predictable. Our data show that it is feasible to scale up a slow rotary countercurrent chromatograph with 1.5 cm I.D. convoluted tubing for industrial use.