Quantitative Determination of Lanatosides in the Hybrid Digitalis ambigua × Digitalis lanata Leaves by HPLC

Abstract

The primary glycosides in the hybrid Digitalis ambigua × Digitalis lanata leaves were determined by HPLC and compared with the measurement of lanatosides in D. ambigua and D. lanata leaves. The extract of dry leaf powder with chloroform:ethanol (1:2, v/v) was submitted to Sep‐Pak cartridges prior to HPLC analysis. HPLC was performed on a TSK‐GEL AMIDE‐80 column using acetonitril:water (96:4). The effluent was monitored by ultraviolet (UV) absorption at 220 nm. The quantitation was carried out by the internal standard method. The amount of lanatoside C per 100 mg of dry leaf powder samples of the hybrid D. ambigua × D. lanata and D. lanata were 227.3 µg and 134.4 µg, respectively. The amount of lanatoside C in the hybrid D. ambigua × D. lanata was higher than in D. lanata.

Keywords:

• Hybrid Digitalis ambigua × Digitalis lanata
• Cardiac glycoside
• Primary glycoside
• HPLC

Acknowledgment

The authors thank Mr. Masaru Yamamoto of the Medicinal Plant Garden of Hokuriku University for providing the leaves of Digitalis used in this study.