A new diamide‐type chiral stationary phase for chiral resolution by normal and reversed phase HPLC

Abstract

In this work, a new diamide‐type chiral stationary phase was developed by chemically bonding the N‐stearoyl‐L‐leucine on the aminopropylsilica gel. It has been successfully used for the chiral resolution of the benzoyl‐, dinitrobenzoyl‐, and trifluoroacethyl amino acids in the modes of normal phase, as well as for the underivatized amino acids in the mode of reversed phase. It was found that the hydrogen bonding makes significant contribution to the chiral resolution. It has been demonstrated that the chiral resolution, by the use of diamide‐type chiral stationary phase (CSP), can be achieved without the π–π interaction between the CSP and analytes.

Keywords:

• Chiral stationary phase
• Diamide
• High performance liquid chromatography
• Amino acid
• Amine

Acknowledgments