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Abstract
The separation of six basic compounds was obtained using a mixed mode of hydrophilic interaction and strong cation exchange (SCX), pressurized capillary electrochromatography (pCEC) on a novel pCEC instrument. The effects of organic modifier concentration, ionic strength, and pH value in the buffer on retention behaviors of solutes were investigated. The effects of pressure and voltage on separation also were evaluated. Moreover, the separation result was compared with that in reversed phase pCEC, and the separation mechanism was discussed. A satisfactory reproducibility and stability in the separation system was also achieved in our study. This paper demonstrates the mixed mode pCEC is potentially applicable for separation of basic compounds.