374
Views
16
CrossRef citations to date
0
Altmetric
Original Articles

Chromatographic Separation of Phenolic Compounds from Rapeseed by a Sephadex LH‐20 Column with Ethanol as the Mobile Phase

, &
Pages 2157-2165 | Received 18 Jan 2003, Accepted 24 Feb 2003, Published online: 16 Aug 2006
 

Abstract

Phenolic compounds from rapeseed were chromatographed using an open column filled with Sephadex LH‐20 as the stationary phase and ethanol as the mobile phase. Typically, methanol has been used as the eluent of choice in previous studies, but it was hypothesized that ethanol's lesser polarity would provide better separation of the rapeseed phenolics. In this work, three fractions (I–III) containing phenolic compounds were collected; their characteristics were evaluated qualitatively and quantitatively by UV spectrophotometry, a colorimetric assay, and capillary electrophoresis (CE). Although fraction I possessed the greatest relative proportion of the crude extract (62.2%), fraction III contained the richest source of rapeseed phenolics (176 mg trans‐sinapic acid equivalents g−1). Capillary electrophoresis identified sinapine as the dominant phenolic compound of fraction I and indicated that a number of phenolic compounds were present in fractions II and III. Although free and esterified derivatives of phenolic acids were constituents of fraction II, the electropherogram revealed that sinapic acid was the chief phenolic compound present, based on retention time mapping. Application of ethanol as the eluent, rather than methanol, gave a better baseline separation of the phenolic constituents from rapeseed.

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 583.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.