Profiling of Secreted Proteins from Human Ovarian Cancer Cell Lines by Surface‐Enhanced Laser Desorption Ionization Time‐of‐Flight Mass Spectrometry

Abstract

Surface‐enhanced laser desorption ionization (SELDI) time‐of‐flight mass spectrometry (TOF MS) was used to profile six human ovarian cancer cell lines. Non‐confluent cell cultures were exchanged into serum free medium and allowed to grow for either 24 or 48 hours, at which time the medium was collected and analyzed using a Ciphergen SELDI‐TOF MS and a QSTAR Pulsar QqTOF mass spectrometer fitted with a SELDI source. The spectra showed the presence of several low molecular weight species, as well as differences and similarities in the proteins detected in the media of the six ovarian tumor cell lines. The same species were detected at 24 and 48 hours and reproducible changes in their relative abundances were observed.

Keywords:

• Biomarker detection
• Protein profile
• Cancer

Acknowledgment

By acceptance of this article, the publisher or recipient acknowledges the right of the U.S. Government to retain a nonexclusive, royalty‐free license and to any copyright covering the article. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organization imply endorsement by the U.S. Government. This project has been funded in whole, or in part, with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. NO1‐CO‐12400.