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Original Articles

Optimization of ASE Conditions for the HPLC Determination of Rutin and Isoquercitrin in Sambucus nigra L

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Pages 2381-2397 | Received 02 Feb 2003, Accepted 06 Mar 2003, Published online: 06 Feb 2007
 

Abstract

The accelerated solvent extraction (ASE) procedure has been examined as a sample preparation method for the HPLC analysis of rutin and isoquercitrin in Sambucus nigra L. The experimental extraction parameters (solvent composition, temperature, operation pressure, and static extraction time) have been optimized in order to receive the best recovery of both analytes from the flowers, leaves, and berries of black elder. The ASE recoveries obtained under the optimal extraction conditions were compared with analogous ones obtained by means of the maceration technique.

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