Abstract
In this study, two methods for the determination of chloramphenicol (CAP) residues in chicken muscle and milk, are proposed. Chromatographic determination was carried out by reversed phase HPLC with a UV photodiode array detector. Confirmation of CAP was performed by comparison of UV spectra. Limit of detection was 0.4 ng g−1 in milk and 2 ng g−1 in muscle samples. The methods were validated and the mean recovery was 78.9% with CV of 9.36% for milk samples, and 86.8% with CV of 7.87% for chicken muscle. Furthermore, the effects of different storage temperatures on the stability of CAP were tested. The results showed a high stability in muscle during refrigeration and freezing storage conditions, but milk freezing produced significant losses of CAP.
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Acknowledgment
This research was supplied by the Spanish Health Research Foundation 97/0797.