Preparative Chiral Chromatography of trans‐Stilbene Oxide Using Cellulose tris(Phenylcarbamate), Chiralcel^® OC, as Stationary Phase

Abstract

Two approaches were studied to improve the productivity of preparative chiral separation. For a model case, trans‐stilbene oxide as a racemate and cellulose tris(phenylcarbamate), “CHIRALCEL^® OC,” as a stationary phase were chosen. The productivity per unit CSP was improved as much as 52% by connecting two different sizes of columns (10 cm ID × 50 cm L and 5 cm ID × 50 cm L) in series. Further improvement may be possible by adjusting the eluent temperature several degrees lower than the jacket temperature of the columns. Preparative separation conditions regarding composition of mobile phase, separation temperature, loading amount of racemic substance, positions of fractions to be taken, and so on were also studied.

Keywords:

• Resolution
• Chiral separation
• High performance liquid chromatography
• HPLC
• Chiral stationary phase
• Coating

Acknowledgment

We wish to thank Dr. T. Shibata for his valuable suggestions.