![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A simple HPLC procedure for the identification and quantitation of phytochelatins (PCs) in plant tissue extracts is described. The method, which does not require a derivatization step utilizes only 20 µL of sample volume. Linear quantitative response curve was generated for phytochelatin 3 (PC3) over a concentration range of 1.33 µmol/L–6.66 mmol/L. Linear regression analysis of the standard curve exhibited correlation coefficient of 0.996. Limit of detection (LOD) and limit of quantitation (LOQ) values were 0.1 and 0.5 µmol, respectively. Phytochelatin 3 recovery using this method was relatively high (above 85%). Intra‐assay and inter‐assay precision studies reflected a high level of reliability and reproducibility of the method. The applicability of the method for the quantitation of a wide range of PC concentrations in plant tissue extracts was demonstrated successfully.
Acknowledgments
The authors would like to thank Dr. Ahmed Al‐Oqlah and Miss Noor Shunnaq for collecting the plant samples, and to Dr. Daniel von Deutsch for his technical assistance. This work was supported, in part, by NASA grant NCC9‐112, NIH grant RCRII 2P20RR11104‐08. Special thanks to the Jordanian‐American Commission for Educational Exchange (JACEE), the Fulbright Foundation, Philadelphia University, and Morehouse School of Medicine for providing Dr. Imad K. Abukhalaf with the opportunity to spend one academic year at Philadelphia University.